1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA
SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 52.1 % (839 of 1611) | 52.6 % (441 of 839) | 42.8 % (270 of 631) | 90.8 % (128 of 141) |
Backbone | 75.4 % (602 of 798) | 85.6 % (232 of 271) | 63.8 % (254 of 398) | 89.9 % (116 of 129) |
Sidechain | 37.5 % (353 of 941) | 36.8 % (209 of 568) | 36.6 % (132 of 361) | 100.0 % (12 of 12) |
Aromatic | 3.6 % (6 of 168) | 4.8 % (4 of 84) | 0.0 % (0 of 82) | 100.0 % (2 of 2) |
Methyl | 38.8 % (52 of 134) | 52.2 % (35 of 67) | 25.4 % (17 of 67) |
1. MLU1-BOX binding protein
SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHHTemperature 288 (±1.0) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MLU1-BOX binding protein | [U-15N] | 0.8 ~ 1.3 mM |
Temperature 288 (±1.0) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | MLU1-BOX binding protein | [U-15N;U-13C] | 0.8 ~ 1.3 mM |
Temperature 288 (±1.0) K, pH 7.6 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | MLU1-BOX binding protein | 0.8 ~ 1.3 mM |