1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from S. cerevisiae in Both Its Free and DNA Bound Forms, and 1H Assignments of the Free DNA
SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHH
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 39.9 % (693 of 1738) | 40.6 % (392 of 966) | 29.3 % (185 of 631) | 82.3 % (116 of 141) |
Backbone | 56.6 % (503 of 889) | 60.2 % (218 of 362) | 42.5 % (169 of 398) | 89.9 % (116 of 129) |
Sidechain | 23.1 % (226 of 977) | 28.8 % (174 of 604) | 14.4 % (52 of 361) | 0.0 % (0 of 12) |
Aromatic | 3.1 % (6 of 194) | 3.6 % (4 of 110) | 2.4 % (2 of 82) | 0.0 % (0 of 2) |
Methyl | 29.7 % (41 of 138) | 40.8 % (29 of 71) | 17.9 % (12 of 67) |
1. MLU1-BOX binding protein
SNQIYSARYS GVDVYEFIHS TGSIMKRKKD DWVNATHILK AANFAKAKRT RILEKEVLKE THEKVQGGFG KYQGTWVPLN IAKQLAEKFS VYDQLKPLFD FTQTDGSASP PPAPKHHHAS KVDKLAAALE HHHHH2. DNA binding domain of Mbp1
TTTGACGCGT CAATemperature 288 (±1) K, pH 7.6 (±0.2), Details Sample 1 is a complex of the DNA binding domain of Mbp1 (residues 2-124) and a 12 base pair DNA duplex (TTTGACGCGTCAA)2 (MBC12T)containing the Mbp1 recognition site Mbp1(124) was expressed in e.coli as a C-terminal hexaHis fusion protein containing a seven-residue linked. For 15N labelled and 15N/13C labelled protein, the growth medium was replaced by M9 minimal medium supplemented with (15NH4)2SO4 (1gL-1) and 12C or 13C6 D-glucose (1gL-1) as the sole nitrogen and carbon sources.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MLU1-BOX binding protein | [U-15N; U-13C] | 0.8 mM | |
2 | DNA binding domain of Mbp1 | 1.0 ~ 1.68 mM | ||
3 | Na phosphate | 40 mM | ||
4 | NaCl | 110 mM | ||
5 | D2O | 10 % | ||
6 | H2O | 90 % |
Temperature 288 (±1) K, pH 7.6 (±0.2), Details Sample 2 is a complex of the DNA binding domain of Mbp1 (residues 2-124) and a 12 base pair DNA duplex (TTTGACGCGTCAA)2 (MBC12T)containing the Mbp1 recognition site Mbp1(124) was expressed in e.coli as a C-terminal hexaHis fusion protein containing a seven-residue linked. For 15N labelled and 15N/13C labelled protein, the growth medium was replaced by M9 minimal medium supplemented with (15NH4)2SO4 (1gL-1) and 12C or 13C6 D-glucose (1gL-1) as the sole nitrogen and carbon sources.
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | MLU1-BOX binding protein | 0.5 mM | ||
8 | DNA binding domain of Mbp1 | 1.0 ~ 1.68 mM | ||
9 | Na phosphate | 40 mM | ||
10 | NaCl | 110 mM | ||
11 | D2O | 10 % | ||
12 | H2O | 90 % |