Sequential Assignment and Solution Secondary Structure of Doubly Labelled Ribonuclease Sa
DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG YYHEYTVITP GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTC
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS7:SG | 1:CYS96:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.4 % (838 of 1055) | 98.7 % (530 of 537) | 50.4 % (212 of 421) | 99.0 % (96 of 97) |
Backbone | 85.6 % (483 of 564) | 99.5 % (193 of 194) | 71.8 % (201 of 280) | 98.9 % (89 of 90) |
Sidechain | 64.1 % (371 of 579) | 98.3 % (337 of 343) | 11.8 % (27 of 229) | 100.0 % (7 of 7) |
Aromatic | 52.9 % (54 of 102) | 96.1 % (49 of 51) | 9.8 % (5 of 51) | |
Methyl | 54.7 % (58 of 106) | 100.0 % (53 of 53) | 9.4 % (5 of 53) |
1. RNAse-SA
DVSGTVCLSA LPPEATDTLN LIASDGPFPY SQDGVVFQNR ESVLPTQSYG YYHEYTVITP GARTRGTRRI ITGEATQEDY YTGDHYATFS LIDQTCTemperature 303.2 (±0.1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RNAse-SA | 1.5 ~ 2.0 mM | ||
2 | H2O | 90 % | ||
3 | D2O | 10 % |
Temperature 303.2 (±0.1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | RNAse-SA | 1.5 ~ 2.0 mM | ||
5 | D2O | 100 % |
Temperature 303.2 (±0.1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | RNAse-SA | [U-15N] | 1.5 ~ 2.0 mM | |
7 | H2O | 90 % | ||
8 | D2O | 10 % |