Assignment of 1H, 13C and 15N Signals of the DNase Domain of Colicin E9
MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 59.7 % (945 of 1584) | 54.0 % (457 of 846) | 60.2 % (363 of 603) | 92.6 % (125 of 135) |
Backbone | 92.0 % (721 of 784) | 91.5 % (247 of 270) | 91.3 % (356 of 390) | 95.2 % (118 of 124) |
Sidechain | 36.6 % (337 of 922) | 36.5 % (210 of 576) | 35.8 % (120 of 335) | 63.6 % (7 of 11) |
Aromatic | 3.8 % (4 of 104) | 3.8 % (2 of 52) | 0.0 % (0 of 50) | 100.0 % (2 of 2) |
Methyl | 27.7 % (26 of 94) | 38.3 % (18 of 47) | 17.0 % (8 of 47) |
1. DNase domain of colicin E9
MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGKTemperature 293 (±1) K, pH 6.2 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNase domain of colicin E9 | [U-99% 13C; U-99% 15N] | 2.0 mM | |
2 | sodium phosphate | 25.0 mM | ||
3 | H20 | 90 % | ||
4 | D20 | 10 % | ||
5 | sodium azide | 0.01 % |
Temperature 293 (±1) K, pH 6.2 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DNase domain of colicin E9 | [U-99% 15N] | 1.5 mM | |
7 | sodium phosphate | 50 mM | ||
8 | H20 | 90 % | ||
9 | D20 | 10 % | ||
10 | sodium azide | 0.01 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.5 % (1379 of 3168) | 39.5 % (668 of 1692) | 44.2 % (533 of 1206) | 65.9 % (178 of 270) |
Backbone | 65.4 % (1025 of 1568) | 65.2 % (352 of 540) | 64.5 % (503 of 780) | 68.5 % (170 of 248) |
Sidechain | 28.0 % (516 of 1844) | 27.5 % (317 of 1152) | 28.5 % (191 of 670) | 36.4 % (8 of 22) |
Aromatic | 6.7 % (14 of 208) | 6.7 % (7 of 104) | 4.0 % (4 of 100) | 75.0 % (3 of 4) |
Methyl | 23.9 % (45 of 188) | 30.9 % (29 of 94) | 17.0 % (16 of 94) |
1. DNase domain of colicin E9
MESKRNKPGK ATGKGKPVGD KWLDDAGKDS GAPIPDRIAD KLRDKEFKSF DDFRKAVWEE VSKDPELSKN LNPSNKSSVS KGYSPFTPKN QQVGGRKVYE LHHDKPISQG GEVYDMDNIR VTTPKRHIDI HRGKTemperature 293 (±1) K, pH 6.2 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNase domain of colicin E9 | [U-99% 13C; U-99% 15N] | 2.0 mM | |
2 | sodium phosphate | 25.0 mM | ||
3 | H20 | 90 % | ||
4 | D20 | 10 % | ||
5 | sodium azide | 0.01 % |
Temperature 293 (±1) K, pH 6.2 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | DNase domain of colicin E9 | [U-99% 15N] | 1.5 mM | |
7 | sodium phosphate | 50 mM | ||
8 | H20 | 90 % | ||
9 | D20 | 10 % | ||
10 | sodium azide | 0.01 % |