Chemical Shifts (1H, 13C, 15N) and J-couplings (3JHNHA, 3JNHB, 3JHAHB) for the N-terminal Domain of E. coli DnaB Helicase, DnaB(24-136)
MKVPPHSIEA EQSVLGGLML DNERWDDVAE RVVADDFYTR PHRHIFTEMA RLQESGSPID LITLAESLER QGQLDSVGGF AYLAELSKNT PSAANISAYA DIVRERAVVR EMIS
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 93.0 % (1196 of 1286) | 94.9 % (631 of 665) | 90.1 % (454 of 504) | 94.9 % (111 of 117) |
Backbone | 94.4 % (636 of 674) | 96.5 % (221 of 229) | 92.9 % (312 of 336) | 94.5 % (103 of 109) |
Sidechain | 91.8 % (661 of 720) | 94.0 % (410 of 436) | 88.0 % (243 of 276) | 100.0 % (8 of 8) |
Aromatic | 75.6 % (59 of 78) | 92.3 % (36 of 39) | 57.9 % (22 of 38) | 100.0 % (1 of 1) |
Methyl | 100.0 % (136 of 136) | 100.0 % (68 of 68) | 100.0 % (68 of 68) |
1. DnaB helicase
MKVPPHSIEA EQSVLGGLML DNERWDDVAE RVVADDFYTR PHRHIFTEMA RLQESGSPID LITLAESLER QGQLDSVGGF AYLAELSKNT PSAANISAYA DIVRERAVVR EMISTemperature 305 (±1) K, pH 7.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DnaB helicase | {U-98% 15N} | 2.2 mM | |
2 | sodium phosphate | 20 mM | ||
3 | EDTA | 1 mM | ||
4 | sodiumn azide | 0.02 % w/v |
Temperature 305 (±1) K, pH 7.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | DnaB helicase | {U-98% 13C, U-98% 15N} | 2.2 mM | |
6 | sodium phosphate | 20 mM | ||
7 | EDTA | 1 mM | ||
8 | sodiumn azide | 0.02 % w/v |
Temperature 305 (±1) K, pH 7.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | DnaB helicase | {U-10% 13C, U-98% 15N} | 2.2 mM | |
10 | sodium phosphate | 20 mM | ||
11 | EDTA | 1 mM | ||
12 | sodiumn azide | 0.02 % w/v |