Backbone and Sequence-Specific Assignment of Three Forms of the Lipoate-Containing H-protein of the Glycine Decarboxylase Complex.
SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA H
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | amide | sing | 1:LYS63:NZ | 2:LPA1:CO |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 33.0 % (287 of 871) | 22.5 % (167 of 741) | 92.3 % (120 of 130) |
Backbone | 64.7 % (253 of 391) | 49.6 % (133 of 268) | 97.6 % (120 of 123) |
Sidechain | 7.1 % (34 of 480) | 7.2 % (34 of 473) | 0.0 % (0 of 7) |
Aromatic | 0.0 % (0 of 51) | 0.0 % (0 of 49) | 0.0 % (0 of 2) |
Methyl | 8.1 % (6 of 74) | 8.1 % (6 of 74) |
1. glycine cleavage system H-protein
SNVLDGLKYA PSHEWVKHEG SVATIGITDH AQDHLGEVVF VELPEPGVSV TKGKGFGAVE SVKATSDVNS PISGEVIEVN TGLTGKPGLI NSSPYEDGWM IKIKPTSPDE LESLLGAKEY TKFCEEEDAA HTemperature 291 (±1) K, pH 5.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | glycine cleavage system H-protein | [U-99% 15N] | 1.3 mM | |
2 | potassium phosphate | 50 mM | ||
3 | EDTA | 0.1 mM | ||
4 | NaN3 | 0.2 % | ||
5 | D2O | 10 % | ||
6 | H2O | 90 % |