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BMRB: 4373

1FHO

1H, 15N, and 13C Resonance Assignment of the PH Domain from C. elegans UNC-89

Authors

Blomberg, N., Sattler, M., Nilges, M.

Assembly

UNC-89 PH domain

Entity

1. UNC-89 PH domain (polymer), 112 monomers, 13379.75 Da Detail

GRIIRHDAFQ VWEGDEPPKL RYVFLFRNKI MFTEQDASTS PPSYTHYSSI RLDKYNIRQH TTDEDTIVLQ PQEPGLPSFR IKPKDFETSE YVRKAWLRDI AEEQEKYAAE RD

Formula weight

13379.75 Da

Source organism

Caenorhabditis elegans

Exptl. method

Refine. method

Automated NOE assignment,Torsion angle dynamics, Cartesian simulated annealing.

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 70.1 %, Completeness (bb): 76.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.1 % (965 of 1376)	82.8 % (601 of 726)	49.1 % (263 of 536)	88.6 % (101 of 114)
Backbone	76.1 % (499 of 656)	91.3 % (200 of 219)	60.1 % (200 of 333)	95.2 % (99 of 104)
Sidechain	67.6 % (560 of 829)	79.1 % (401 of 507)	50.3 % (157 of 312)	20.0 % (2 of 10)
Aromatic	45.8 % (66 of 144)	87.5 % (63 of 72)	1.4 % (1 of 70)	100.0 % (2 of 2)
Methyl	87.8 % (86 of 98)	91.8 % (45 of 49)	83.7 % (41 of 49)

1. PH domain from C. elegans UNC-89 protein

GRIIRHDAFQ VWEGDEPPKL RYVFLFRNKI MFTEQDASTS PPSYTHYSSI RLDKYNIRQH TTDEDTIVLQ PQEPGLPSFR IKPKDFETSE YVRKAWLRDI AEEQEKYAAE RD

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

LACS Plot; CA

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.14 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 1FHO, Strand ID: A Detail

Release date

2000-12-13

Citation 1

1H, 15N, and 13C Resonance Assignment of the PH Domain from C. elegans UNC-89
Blomberg, N., Sattler, M., Nilges, M.
J. Biomol. NMR (1999), 15, 269-270, PubMed , DOI:

Show more 2 citaions

Citation 2

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 , Abstract

Citation 3

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Hide non-primary 2 citaions

Related entities 1. UNC-89 PH domain, : 1 : 1 : 8 entities Detail

Interaction partners 1. UNC-89 PH domain, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 11 experiments Detail

1 1H-15N HSQC NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

2 1H-15N HSQC TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

3 CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

4 CBCANH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

5 HNCA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

6 HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

7 HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

8 HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

9 1H-13C HMQC NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

10 2D 1H-1H NOESY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

11 HNHA-J

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Temperature 303 (±1) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	PH domain from C. elegans UNC-89 protein	[U-13C; U-15N]		0.6 ~ 0.8 mM
2	Pi			5 mM
3	NaCl			50 mM
4	d6-DMSO			16 %

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