Letter to the Editor:Sequence-specific 1H, 13C, and 15N assignments for the third EH domain of Eps15 (EH3)
KRKTWVVSPA EKAKYDEIFL KTDKDMDGFV SGLEVREIFL KTGLPSTLLA HIWSLCDTKD CGKLSKDQFA LAFHLISQKL IKGIDPPHVL TPEMIPPSDR ASLQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.8 % (1136 of 1251) | 90.7 % (592 of 653) | 90.7 % (450 of 496) | 92.2 % (94 of 102) |
Backbone | 91.1 % (556 of 610) | 92.2 % (190 of 206) | 90.2 % (277 of 307) | 91.8 % (89 of 97) |
Sidechain | 90.8 % (672 of 740) | 90.2 % (403 of 447) | 91.7 % (264 of 288) | 100.0 % (5 of 5) |
Aromatic | 90.4 % (85 of 94) | 93.6 % (44 of 47) | 86.7 % (39 of 45) | 100.0 % (2 of 2) |
Methyl | 96.8 % (120 of 124) | 96.8 % (60 of 62) | 96.8 % (60 of 62) |
1. third EH domain of Eps15
KRKTWVVSPA EKAKYDEIFL KTDKDMDGFV SGLEVREIFL KTGLPSTLLA HIWSLCDTKD CGKLSKDQFA LAFHLISQKL IKGIDPPHVL TPEMIPPSDR ASLQTemperature 298 K, pH 7.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | third EH domain of Eps15 | [U-13C; U-15N] | 1.0 mM | |
2 | d-tris | 10 mM | ||
3 | KCl | 100 mM | ||
4 | NaN3 | 2 mM | ||
5 | APMSF | 0.010 mM | ||
6 | d-DTT | 0.1 ~ 40.0 mM | ||
7 | D2O | 10 % | ||
8 | H2O | 90 % |
Temperature 298 K, pH 7.8
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | third EH domain of Eps15 | [U-13C; U-15N] | 1.0 mM | |
10 | d-tris | 10 mM | ||
11 | KCl | 100 mM | ||
12 | NaN3 | 2 mM | ||
13 | APMSF | 0.010 mM | ||
14 | d-DTT | 0.1 ~ 40.0 mM | ||
15 | D2O | 99.9 % |