BMRBj - BMREntryMaster

Found 1 Document (1.861 seconds)

BMRB: 4401

1BLQ

Structure and Interaction Site of the Regulatory Domain of Troponin-C when Complexed with the 96-148 Region of Troponin-I

Authors

McKay, R.T., Pearlstone, J.R., Corson, D.C., Gagne, S.M., Smillie, L.B., Sykes, B.D.

Assembly

skeletal N-troponin C

Entity

1. skeletal N-troponin C (polymer), 90 monomers, 9977.024 Da Detail

ASMTDQQAEA RAFLSEEMIA EFKAAFDMFD ADGGGDISTK ELGTVMRMLG QNPTKEELDA IIEEVDEDGS GTIDFEEFLV MMVRQMKEDA

2. sTnI115-131 (polymer), 53 monomers, 6149.311 Da Detail

NQKLFDLRGK FKRPPLRRVR MSADAMLKAL LGSKHKVAMD LRANLKQVKK EDT

Total weight

16126.336 Da

Max. entity weight

9977.024 Da

Source organism

Gallus gallus , Oryctolagus cuniculus

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 64.3 %, Completeness: 52.7 %, Completeness (bb): 61.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.7 % (877 of 1664)	52.1 % (455 of 874)	52.0 % (333 of 641)	59.7 % (89 of 149)
Backbone	61.5 % (524 of 852)	63.0 % (184 of 292)	60.5 % (254 of 420)	61.4 % (86 of 140)
Sidechain	45.5 % (430 of 946)	46.6 % (271 of 582)	43.9 % (156 of 355)	33.3 % (3 of 9)
Aromatic	19.0 % (16 of 84)	26.2 % (11 of 42)	11.9 % (5 of 42)
Methyl	58.5 % (83 of 142)	57.7 % (41 of 71)	59.2 % (42 of 71)

1. skeletal N-troponin C

ASMTDQQAEA RAFLSEEMIA EFKAAFDMFD ADGGGDISTK ELGTVMRMLG QNPTKEELDA IIEEVDEDGS GTIDFEEFLV MMVRQMKEDA

2. sTnI115-131

NQKLFDLRGK FKRPPLRRVR MSADAMLKAL LGSKHKVAMD LRANLKQVKK EDT

Show sample condition

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.68 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 29 models in PDB: 1BLQ, Strand ID: A Detail

Release date

2005-05-25

Citation 1

Structure and interaction site of the regulatory domain of troponin-C when complexed with the 96-148 region of troponin-I
McKay, R.T., Pearlstone, J.R., Corson, D.C., Gagne, S.M., Smillie, L.B., Sykes, B.D.
Biochemistry (1998), 37, 12419-12430, PubMed 9730814 , DOI 10.1021/bi9809019 , Abstract

Show more 2 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 3

A short recollection on the paper entitled "A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams" by D.S. Garrett, R. Powers, A.M. Gronenborn, and G.M. Clore [J. Magn. Reson. 95 (1991) 214-220]
J. Magn. Reson. (2011), 213, 364-365, PubMed 22152356 , DOI 10.1016/j.jmr.2011.08.009 , Abstract

Hide non-primary 2 citaions

Related entities 1. skeletal N-troponin C, : 106 entities Detail

Related entities 2. sTnI115-131, : 7 : 50 entities Detail

Interaction partners 2. sTnI115-131, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 11 experiments Detail

1 15N-HMQC

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

2 15N-HSQC

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

3 TOCSY-hmqc

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

4 HCCH-TOCSY

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

5 CBCA(CO)NNH

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

6 15N-noesyhsqc

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

7 15N-noesyhmqc

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

8 13C15N-noesy

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

9 HNCO

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

10 HNCA

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

11 HCACO

Instrument

#1 Model Varian Unity-Inova (500 MHz)

#2 Model Varian Unity (600 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	skeletal N-troponin C	[U-98% 13C; U-98% 15N]		0.7 mM
2	KCl			100 mM

Sample #2

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	skeletal N-troponin C	[U-98% 15N; U-88% 2H]		0.7 mM
4	KCl			100 mM

Sample #3

Pressure 1 (±0.1) atm, Temperature 303 (±1) K, pH 6.85 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	skeletal N-troponin C	unlabeled		0.9 mM
6	KCl			100 mM

nullKeywords calcium-binding regulation, contraction, skeletal muscle, troponin C

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Found 1 Document (1.861 seconds)

BMRB: 4401

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. skeletal N-troponin C, : 106 entities Detail

Related entities 2. sTnI115-131, : 7 : 50 entities Detail

Interaction partners 2. sTnI115-131, : 2 interactors Detail

Experiments performed 11 experiments Detail

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