The Structure of the Transcriptional Antiterminator NusB from Escherichia Coli
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKK
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 87.0 % (1431 of 1645) | 91.8 % (786 of 856) | 79.2 % (509 of 643) | 93.2 % (136 of 146) |
Backbone | 82.6 % (681 of 824) | 93.9 % (261 of 278) | 71.6 % (295 of 412) | 93.3 % (125 of 134) |
Sidechain | 92.0 % (879 of 955) | 90.8 % (525 of 578) | 94.0 % (343 of 365) | 91.7 % (11 of 12) |
Aromatic | 96.4 % (108 of 112) | 96.4 % (54 of 56) | 96.4 % (53 of 55) | 100.0 % (1 of 1) |
Methyl | 98.9 % (178 of 180) | 98.9 % (89 of 90) | 98.9 % (89 of 90) |
1. NusB
MKPAARRRAR ECAVQALYSW QLSQNDIADV EYQFLAEQDV KDVDVLYFRE LLAGVATNTA YLDGLMKPYL SRLLEELGQV EKAVLRIALY ELSKRSDVPY KVAINEAIEL AKSFGAEDSH KFVNGVLDKA APVIRPNKKTemperature 298 (±2) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NusB | [U-15N] | 0.7 ~ 1.2 mM |
Temperature 298 (±2) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | NusB | [U-2H; U-15N] | 0.7 ~ 1.2 mM |
Temperature 298 (±2) K, pH 6.8 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | NusB | [U-15N; U-13C] | 0.7 ~ 1.2 mM |