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BMRB: 4804

1HKO

1H,13C and 15N chemical shifts of bovine ferrous cytochrome b5-N17D

Authors

Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.

Assembly

bovine ferrous cytochrome b5-N17D

Entity

1. ferrous cytochrome b5 (polymer, Thiol state: not present), 82 monomers, 9462.250 Da Detail

AVKYYTLEEI QKHNDSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. HEM (non-polymer), 616.487 Da

Total weight

10078.737 Da

Max. entity weight

9462.25 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.1 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	97.1 % (930 of 958)	99.0 % (491 of 496)	94.4 % (356 of 377)	97.6 % (83 of 85)
Backbone	97.1 % (474 of 488)	97.6 % (164 of 168)	96.7 % (232 of 240)	97.5 % (78 of 80)
Sidechain	95.1 % (519 of 546)	97.3 % (319 of 328)	91.5 % (195 of 213)	100.0 % (5 of 5)
Aromatic	78.7 % (74 of 94)	87.2 % (41 of 47)	69.6 % (32 of 46)	100.0 % (1 of 1)
Methyl	98.8 % (83 of 84)	100.0 % (42 of 42)	97.6 % (41 of 42)

1. ferrous cytochrome b5

AVKYYTLEEI QKHNDSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	ferrous cytochrome b5	[U-90% 13C; U-90% 15N]		2.0 ~ 9.0 mM
2	PROTOPORPHYRIN IX CONTAINING FE			2.0 ~ 9.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	0.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.12 ppm, Outliers: 2 Detail

Release date

2000-11-14

Citation 1

NMR studies of the association of cytochrome b5 with cytochrome c
Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.
Biochemistry (2000), 39, 14025-14039, PubMed 11087350 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. ferrous cytochrome b5, : 1 : 12 : 250 entities Detail

Interaction partners 1. ferrous cytochrome b5, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC