BMRBj - BMREntryMaster

Found 1 Document (1.306 seconds)

BMRB: 4807

1HKO

1H, 13C and 15N chemical shifts of bovine ferric cytochrome b5-N17D

Authors

Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.

Assembly

bovine ferric cytochrome b5-N17D

Entity

1. ferric cytochrome b5 (polymer, Thiol state: not present), 82 monomers, 9462.250 Da Detail

AVKYYTLEEI QKHNDSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. HEM (non-polymer), 616.487 Da

Total weight

10078.737 Da

Max. entity weight

9462.25 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.6 %, Completeness: 31.7 %, Completeness (bb): 51.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.7 % (304 of 958)	26.2 % (130 of 496)	26.0 % (98 of 377)	89.4 % (76 of 85)
Backbone	51.6 % (252 of 488)	52.4 % (88 of 168)	36.7 % (88 of 240)	95.0 % (76 of 80)
Sidechain	10.8 % (59 of 546)	12.8 % (42 of 328)	8.0 % (17 of 213)	0.0 % (0 of 5)
Aromatic	9.6 % (9 of 94)	12.8 % (6 of 47)	6.5 % (3 of 46)	0.0 % (0 of 1)
Methyl	14.3 % (12 of 84)	21.4 % (9 of 42)	7.1 % (3 of 42)

1. ferric cytochrome b5

AVKYYTLEEI QKHNDSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	ferric cytochrome b5	[U-90% 13C; U-90% 15N]		0.8 mM
2	PROTOPORPHYRIN IX CONTAINING FE			0.8 mM

Release date

2000-11-14

Citation 1

NMR studies of the association of cytochrome b5 with cytochrome c
Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.
Biochemistry (2000), 39, 14025-14039, PubMed 11087350 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Hide non-primary 1 citaion

Related entities 1. ferric cytochrome b5, : 1 : 12 : 250 entities Detail

Interaction partners 1. ferric cytochrome b5, : 4 interactors Detail

More details for 4 interactors identified by 4 citations

Experiments performed 4 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4807_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4807_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	298		97.6 (chain: 1, length: 82)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 82, Sequence coverage: 97.6%
- Total number of assignments
  - ¹H chemical shifts: 133
  - ¹³C chemical shifts: 89
  - ¹⁵N chemical shifts: 76
- Completeness of assignments
  - Entity_assemble_ID: 1
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	496	117	23.6
¹³C chemical shifts	377	89	23.6
¹⁵N chemical shifts	88	76	86.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	168	84	50.0
¹³C chemical shifts	164	75	45.7
¹⁵N chemical shifts	80	76	95.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	328	33	10.1
¹³C chemical shifts	213	14	6.6
¹⁵N chemical shifts	8	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	42	8	19.0
¹³C chemical shifts	42	2	4.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	47	3	6.4
¹³C chemical shifts	46	3	6.5
¹⁵N chemical shifts	1	0	0.0

Keywords bovine cytochrome b5, heteronuclear NMR

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Found 1 Document (1.306 seconds)

BMRB: 4807

Sample

Related entities 1. ferric cytochrome b5, : 1 : 12 : 250 entities Detail

Interaction partners 1. ferric cytochrome b5, : 4 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail