BMRB: 4810

1H, 13C and 15N chemical shifts of bovine ferric cytochrome b5 in complex with horse heart ferric cytochrome c

Authors

Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.

Assembly

bovine ferric cytochrome b5 in complex with ferric horse heart cytochrome c

Entity

1. ferric cytochrome b5 (polymer, Thiol state: not present), 82 monomers, 9461.265 Da Detail

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. ferric cytochrome c (polymer, Thiol state: all other bound), 104 monomers, 11701.40 Da Detail

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

3. HEM (non-polymer), 616.487 Da
4. HEC (non-polymer), 618.503 Da

Total weight

22397.654 Da

Max. entity weight

11701.4 Da

Source organism

Bos taurus , Equus caballus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 42.5 %, Completeness: 12.1 %, Completeness (bb): 21.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	12.1 % (268 of 2221)	9.5 % (111 of 1165)	9.3 % (80 of 861)	39.5 % (77 of 195)
Backbone	21.4 % (236 of 1104)	21.9 % (84 of 384)	14.1 % (76 of 540)	42.2 % (76 of 180)
Sidechain	3.0 % (39 of 1285)	3.6 % (28 of 781)	2.0 % (10 of 489)	6.7 % (1 of 15)
Aromatic	5.3 % (10 of 190)	7.4 % (7 of 95)	3.2 % (3 of 93)	0.0 % (0 of 2)
Methyl	0.6 % (1 of 176)	1.1 % (1 of 88)	0.0 % (0 of 88)

1. ferric cytochrome b5

AVKYYTLEEI QKHNNSKSTW LILHYKVYDL TKFLEEHPGG EEVLREQAGG DATENFEDVG HSTDARELSK TFIIGELHPD DR

2. ferric cytochrome c

GDVEKGKKIF VQKCAQCHTV EKGGKHKTGP NLHGLFGRKT GQAPGFTYTD ANKNKGITWK EETLMEYLEN PKKYIPGTKM IFAGIKKKTE REDLIAYLKK ATNE

Show sample condition

Sample

Temperature 298 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	ferric cytochrome b5	[U-90% 13C; U-90% 15N]		0.3 mM
2	ferric cytochrome c			1.2 mM
3	PROTOPORPHYRIN IX CONTAINING FE			0.0 ~ 0.0 mM

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Release date

2000-11-14

Citation 1

NMR studies of the association of cytochrome b5 with cytochrome c
Hom, K., Ma, Q., Wolfe, G., Zhang, H., Storch, E.M., Daggett, V., Basus, V.J., Waskell, L.
Biochemistry (2000), 39, 14025-14039, PubMed 11087350 , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. ferric cytochrome b5, : 1 : 4 : 13 : 12 : 232 entities Detail

Related entities 2. ferric cytochrome c, : 40 entities Detail

Interaction partners 1. ferric cytochrome b5, : 4 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 6 contents Detail