Chemical shift assignments for the isolated N-terminal domain of EPSP synthase
MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 82.7 % (2216 of 2679) | 81.9 % (1128 of 1377) | 84.3 % (889 of 1054) | 80.2 % (199 of 248) |
Backbone | 87.4 % (1238 of 1416) | 86.6 % (427 of 493) | 88.6 % (616 of 695) | 85.5 % (195 of 228) |
Sidechain | 78.9 % (1166 of 1478) | 79.3 % (701 of 884) | 80.3 % (461 of 574) | 20.0 % (4 of 20) |
Aromatic | 13.5 % (17 of 126) | 15.9 % (10 of 63) | 11.1 % (7 of 63) | |
Methyl | 95.5 % (315 of 330) | 93.9 % (155 of 165) | 97.0 % (160 of 165) |
1. N-terminal EPSP synthase
MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVETemperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | null | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13C | DSS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | null | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13C | DSS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | null | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13C | DSS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Atom type | Mol. common name | Atom group | Value | Ref. method | Ref. type | Shift ratio |
---|---|---|---|---|---|---|
1H | DSS | methyl protons | 0.0 ppm | null | direct | 1.0 |
15N | DSS | methyl protons | 0.0 ppm | null | indirect | 0.1013291 |
13C | DSS | methyl protons | 0.0 ppm | null | indirect | 0.2514495 |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Varian Inova - 500 MHz
State isotropic, Temperature 295 (±1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | N-terminal EPSP synthase | [U-15N; U-13C; U-70% 2H] | 1.0 mM | |
2 | Tris | 50 mM | ||
3 | DTT | 1 mM |
Properties
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneContent subtype: bmr4854_3.str
Assigned chemical shifts
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100 MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNLLDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMR | |||||||||||||||||| | | |||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| M.SLTLQPIARVDGTINLPG.K.V..RALLLAALAHGKTVLTNLLDSDDVRH.LNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMR -------110-------120-------130-------140-------150-------160-------170-------180-------190-------200 PLAAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGGNVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPY ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| PLAAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGGNVDVDGSVSSQ.LTALLMTAPLAPEDTVIRIKGDLVSKPY -------210-------220-------230-------240 IDITLNLMKTFGVEIENQHYQQFVVKGGQSYQSPGTYLVE |||||||||||||||||||||||||||||||||||||||| IDITLNLMKTFGVEIENQHYQQFVVKGGQSYQSPGTYLVE
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 1377 | 1090 | 79.2 |
13C chemical shifts | 1054 | 867 | 82.3 |
15N chemical shifts | 259 | 195 | 75.3 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 493 | 415 | 84.2 |
13C chemical shifts | 480 | 417 | 86.9 |
15N chemical shifts | 228 | 195 | 85.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 884 | 675 | 76.4 |
13C chemical shifts | 574 | 450 | 78.4 |
15N chemical shifts | 31 | 0 | 0.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 171 | 160 | 93.6 |
13C chemical shifts | 171 | 165 | 96.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 63 | 0 | 0.0 |
13C chemical shifts | 63 | 0 | 0.0 |