BMRB: 4874

Sequence-specific assignment of the PAH2 domain of Sin3B free and complexed to Mad1

Authors

Spronk, C.A.E.M., Jansen, J.F.A., Tessari, M., Kaan, A.M., Aelen, J., Vermeulen, M., Stunnenberg, H.G., Vuister, G.W.

Assembly

mSin3B-PAH2 free state

Entity

1. mSin3B-PAH2 free state (polymer, Thiol state: not present), 105 monomers, 12193.36 Da Detail

ESDSVEFNNA ISYVNKIKTR FLDHPEIYRS FLEILHTYQK EQLHTKGRPF RGMSEEEVFT EVANLFRGQE DLLSEFGQFL PEAKRSLFTG NGSAEMNSGQ KNEEK

Formula weight

12193.36 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 43.8 %, Completeness (bb): 81.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	43.8 % (555 of 1266)	21.8 % (145 of 666)	63.4 % (308 of 486)	89.5 % (102 of 114)
Backbone	81.7 % (510 of 624)	52.3 % (112 of 214)	96.1 % (296 of 308)	100.0 % (102 of 102)
Sidechain	18.5 % (137 of 740)	7.3 % (33 of 452)	37.7 % (104 of 276)	0.0 % (0 of 12)
Aromatic	7.9 % (10 of 126)	7.9 % (5 of 63)	7.9 % (5 of 63)
Methyl	7.0 % (6 of 86)	4.7 % (2 of 43)	9.3 % (4 of 43)

1. mSin3B-PAH2 domain

ESDSVEFNNA ISYVNKIKTR FLDHPEIYRS FLEILHTYQK EQLHTKGRPF RGMSEEEVFT EVANLFRGQE DLLSEFGQFL PEAKRSLFTG NGSAEMNSGQ KNEEK

Show sample condition

Sample

Temperature 293 (±1) K, pH 6.5 (±0.1), Details Trace amounts of Sodium azide and protease inhibitors were added for conservation

#	Name	Isotope labeling	Type	Concentration
1	mSin3B-PAH2 domain	[U-100% 13C; U-100% 15N]		1.0 mM
2	Potassium Phosphate			50 mM
3	Potassium Chloride			100 mM

Hide sample codition

LACS Plot; CA

Referencing offset: -0.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: -0.5 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: -0.49 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

Hide chemical shift referencing parameters

Release date

2001-04-25

Citation 1

Letter to the editor: Sequence-specific assignment of the PAH2 domain of Sin3B free and bound to Mad1
Spronk, C.A.E.M., Jansen, J.F.A., Tessari, M., Kaan, A.M., Aelen, J., Lasonder, E., Stunnenberg, H.G., Vuister, G.W.
J. Biomol. NMR (2001), 19, 377-378, PubMed , DOI:

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. mSin3B-PAH2 free state, : 1 : 5 : 34 entities Detail

Interaction partners 1. mSin3B-PAH2 free state, : 9 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail