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Backbone Assignments for Af19
Authors
Pakhomova, O.N., Cui, Y., Zwieb, C., Hinck, A.P.
Assembly
Signal Recognition Particle Protein SRP19
Entity
1. Signal Recognition Particle Protein SRP19 (polymer, Thiol state: not present), 104 monomers, 12372.43 Da Detail

MKESVVWTVN LDSKKSRAEG RRIPRRFAVP NVKLHELVEA SKELGLKFRA EEKKYPKSWW EEGGRVVVEK RGTKTKLMIE LARKIAEIRE QKREQKKDKK KKKK


Formula weight
12372.43 Da
Source organism
Archaeoglobus fulgidus
Exptl. method
NMR
Refine. method
Distance geometry/simulated annealing protocol
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 92.3 %, Completeness: 84.2 %, Completeness (bb): 90.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All84.2 % (1126 of 1337)83.3 % (602 of 723)85.4 % (432 of 506)85.2 % (92 of 108)
Backbone90.9 % (562 of 618)90.0 % (189 of 210)91.9 % (282 of 307)90.1 % (91 of 101)
Sidechain80.1 % (655 of 818)80.5 % (413 of 513)80.9 % (241 of 298)14.3 % (1 of 7)
Aromatic10.3 % (7 of 68)17.6 % (6 of 34) 0.0 % (0 of 31)33.3 % (1 of 3)
Methyl100.0 % (98 of 98)100.0 % (49 of 49)100.0 % (49 of 49)

1. Archaeglobus fulgidus SRP19

MKESVVWTVN LDSKKSRAEG RRIPRRFAVP NVKLHELVEA SKELGLKFRA EEKKYPKSWW EEGGRVVVEK RGTKTKLMIE LARKIAEIRE QKREQKKDKK KKKK

Sample #1

Temperature 300 (±1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
1Archaeglobus fulgidus SRP19[U-99% 13C; U-98% 15N]1.8 ~ 2.2 mM
2NaCl0.050 M
3KH2PO40.025 M
4D2O99.88 %
Sample #2

Temperature 300 (±1) K, pH 6.0 (±0.1)


#NameIsotope labelingTypeConcentration
5Archaeglobus fulgidus SRP19[U-98% 15N]1.8 ~ 2.2 mM
6NaCl0.050 M
7KH2PO40.025 M
8D2O5 %
9H2O95 %

LACS Plot; CA
Referencing offset: -0.14 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.14 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.08 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1KVV, Strand ID: A Detail


Release date
2001-06-28
Citation 1
Sequence-specific 1H, 13C and 15N signal assignments and secondary structure of archaeoglobusfulgidus SRP19
Pakhomova, O.N., Cui, Y., Zwieb, C., Hinck, A.P.
J. Biomol. NMR (2001), 20, 187-188, PubMed 11495253 , DOI: ,
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 ,
Citation 3
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 ,
Related entities 1. Signal Recognition Particle Protein SRP19, : 1 : 1 : 1 : 45 entities Detail
Experiments performed 9 experiments Detail
nullKeywords RNA-binding protein, signal recogntion particle, SRP