BMRB: 4980

1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease

Authors

Crescenzi, O., Carotenuto, A., D'Ursi, A.M., Tancredi, T., D'Alessio, G., Avitabile, F., Picone, D.

Assembly

bovine seminal ribonuclease monomer (N67D mutant)

Entity

1. bovine seminal ribonuclease monomer (polymer, Thiol state: all disulfide bound), 125 monomers, 13742.63 Da Detail

MKESAAAKFE RQHMDSGNSP SSSSNYCNLM MCCRKMTQGK CKPVNTFVHE SLADVKAVCS QKKVTCKDGQ TNCYQSKSTM RITDCRETGS SKYPNCAYKT TQVEKHIIVA CGGKPSVPVH FDASV

2. GLUTATHIONE (non-polymer), 307.323 × 2 Da

Total weight

14357.275 Da

Max. entity weight

13742.63 Da

Entity Connection

disulfide 6 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS32:SG	2:GSH1:SG2
2	disulfide	sing	1:CYS33:SG	2:GSH1:SG2
3	disulfide	sing	1:CYS27:SG	1:CYS85:SG
4	disulfide	sing	1:CYS41:SG	1:CYS96:SG
5	disulfide	sing	1:CYS59:SG	1:CYS111:SG
6	disulfide	sing	1:CYS66:SG	1:CYS73:SG

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS, ENERGY MINIMIZATION

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 86.4 %, Completeness (bb): 97.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	86.4 % (747 of 865)	85.0 % (623 of 733)	93.9 % (124 of 132)
Backbone	97.0 % (360 of 371)	97.2 % (244 of 251)	96.7 % (116 of 120)
Sidechain	78.5 % (388 of 494)	78.8 % (380 of 482)	66.7 % (8 of 12)
Aromatic	94.9 % (37 of 39)	94.9 % (37 of 39)
Methyl	100.0 % (47 of 47)	100.0 % (47 of 47)

1. bovine seminal ribonuclease monomer

MKESAAAKFE RQHMDSGNSP SSSSNYCNLM MCCRKMTQGK CKPVNTFVHE SLADVKAVCS QKKVTCKDGQ TNCYQSKSTM RITDCRETGS SKYPNCAYKT TQVEKHIIVA CGGKPSVPVH FDASV

Show sample condition

Sample

Temperature 300 (±0.2) K, pH 5.65 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	bovine seminal ribonuclease monomer	[U-97% 15N]		2.0 mM
2	GLUTATHIONE			4.0 mM

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Protein Blocks Logo

Calculated from 16 models in PDB: 1QWQ, Strand ID: A Detail

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Release date

2001-03-24

Citation 1

Letter to the Editor: 1H and 15N sequential assignment and secondary structure of the monomeric N67D mutant of bovine seminal ribonuclease
Crescenzi, O., Carotenuto, A., D'Ursi, A.M., Tancredi, T., D'Alessio, G., Avitabile, F., Picone, D.
J. Biomol. NMR (2001), 20, 289-290, PubMed , DOI:

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Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 3

No title is available
null

Citation 4

Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 , Abstract

Citation 5

No title is available
null

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Related entities 1. bovine seminal ribonuclease monomer, : 1 : 5 : 236 entities Detail

Experiments performed 10 experiments Detail