Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 0:CYS5:SG | 1:CYS56:SG |
2 | disulfide | sing | 0:CYS41:SG | 1:CYS66:SG |
3 | disulfide | sing | 0:CYS48:SG | 1:CYS86:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.2 % (1004 of 1033) | 97.6 % (531 of 544) | 96.2 % (385 of 400) | 98.9 % (88 of 89) |
Backbone | 97.8 % (499 of 510) | 98.8 % (171 of 173) | 96.9 % (246 of 254) | 98.8 % (82 of 83) |
Sidechain | 97.0 % (587 of 605) | 97.0 % (360 of 371) | 96.9 % (221 of 228) | 100.0 % (6 of 6) |
Aromatic | 95.3 % (82 of 86) | 90.7 % (39 of 43) | 100.0 % (42 of 42) | 100.0 % (1 of 1) |
Methyl | 100.0 % (72 of 72) | 100.0 % (36 of 36) | 100.0 % (36 of 36) |
1. beta2-glycoprotein I
TKASCKLPVK KATVVYQGER VKIQEKFKNG MLHGDKVSFF CKNKEKKCSY TEDAQCIDGT IEVPKCFKEH SSLAFWKTDA SDVKPCTemperature 298 (±0.1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | beta2-glycoprotein I | [U-15N] | 1.0 mM |
Temperature 298 (±0.1) K, pH 6.0 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
2 | beta2-glycoprotein I | [U-15N; U-13C] | 1.0 mM |