BMRB: 5066

1H and 15N Chemical Shift Assignments for the Alpha-domain of Mouse Metallothionein-3

Authors

Oz, G., Zangger, K., Armitage, I.M.

Assembly

metallothionein-3

Entity

1. Metallothionein-3 (polymer, Thiol state: all other bound), 68 monomers, 7009.224 Da Detail

MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE AEKCSCCQ

2. CD (non-polymer), 112.411 × 7 Da

Total weight

7796.101 Da

Max. entity weight

7009.224 Da

Entity Connection

metal coordination 16 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS51:SG	2:CD1:CD
2	metal coordination	sing	1:CYS64:SG	2:CD1:CD
3	metal coordination	sing	1:CYS66:SG	2:CD1:CD
4	metal coordination	sing	1:CYS67:SG	2:CD1:CD
5	metal coordination	sing	1:CYS34:SG	2:CD1:CD
6	metal coordination	sing	1:CYS35:SG	2:CD1:CD
7	metal coordination	sing	1:CYS45:SG	2:CD1:CD
8	metal coordination	sing	1:CYS49:SG	2:CD1:CD
9	metal coordination	sing	1:CYS38:SG	2:CD1:CD
10	metal coordination	sing	1:CYS42:SG	2:CD1:CD
11	metal coordination	sing	1:CYS45:SG	2:CD1:CD
12	metal coordination	sing	1:CYS67:SG	2:CD1:CD
13	metal coordination	sing	1:CYS35:SG	2:CD1:CD
14	metal coordination	sing	1:CYS37:SG	2:CD1:CD
15	metal coordination	sing	1:CYS38:SG	2:CD1:CD
16	metal coordination	sing	1:CYS51:SG	2:CD1:CD

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

HYBRID DISTANCE GEOMETRY-DYNAMICAL SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 57.4 %, Completeness: 54.3 %, Completeness (bb): 56.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	54.3 % (232 of 427)	54.3 % (196 of 361)	54.5 % (36 of 66)
Backbone	56.9 % (115 of 202)	57.2 % (79 of 138)	56.3 % (36 of 64)
Sidechain	52.0 % (117 of 225)	52.5 % (117 of 223)	0.0 % (0 of 2)
Methyl	63.6 % (7 of 11)	63.6 % (7 of 11)

1. Metallothionein-3

MDPETCPCPT GGSCTCSDKC KCKGCKCTNC KKSCCSCCPA GCEKCAKDCV CKGEEGAKAE AEKCSCCQ

Show sample condition

Sample

Temperature 283 (±0.1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Metallothionein-3	[U-15N]		1.0 ~ 3.0 mM
2	Tris-HCl	[U-98% 2H]		20 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1JI9, Strand ID: A Detail

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Release date

2001-10-16

Citation 1

Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3
Oz, G., Zangger, K., Armitage, I.M.
Biochemistry (2001), 40, 11433-11441, PubMed 11560491 , Abstract

Show more 1 citaion

Citation 2

1H, 13C and 15N chemical shift referencing in biomolecular NMR
Wishart, D.S., Bigam, C.G., Yao, J., Abildgaard, F., Dyson, H.J., Oldfield, E., Markley, J.L., Sykes, B.D.
J. Biomol. NMR (1995), 6, 135-140, PubMed 8589602 , Abstract

Hide non-primary 1 citaion

Related entities 1. Metallothionein-3, : 2 : 84 entities Detail

Interaction partners 1. Metallothionein-3, : 2 interactors Detail

Experiments performed 5 experiments Detail