BMRBj - BMREntryMaster

Found 1 Document (1.32 seconds)

BMRB: 5231

1SLM

Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor

Authors

Sahasrabudhe, P.V., Stockman, B.J.

Assembly

Stromelysin complex with a ligand

Entity

1. stromelysin (polymer, Thiol state: not present), 173 monomers, 19395.33 Da Detail

FRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PET

2. beta-hydroxamate inhibitor (non-polymer), 1 monomers, na Da

Formula weight

19395.33 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.6 %, Completeness: 56.5 %, Completeness (bb): 82.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.5 % (1127 of 1994)	48.0 % (492 of 1025)	62.2 % (496 of 798)	81.3 % (139 of 171)
Backbone	82.2 % (830 of 1010)	78.3 % (270 of 345)	83.4 % (422 of 506)	86.8 % (138 of 159)
Sidechain	38.0 % (435 of 1144)	32.6 % (222 of 680)	46.9 % (212 of 452)	8.3 % (1 of 12)
Aromatic	2.6 % (6 of 232)	3.4 % (4 of 116)	1.8 % (2 of 113)	0.0 % (0 of 3)
Methyl	42.9 % (78 of 182)	47.3 % (43 of 91)	38.5 % (35 of 91)

1. stromelysin

FRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PET

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	stromelysin	[U-100% 13C; U-100% 15N]	0.9 mM
2	beta-hydroxamate inhibitor		1 mM
3	d-imidazole		10 mM
4	CaCl2		2.5 mM
5	ZnCl2		5 uM
6	NaN3		0.02 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.7 ppm	internal	direct	0.0
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.7 ppm	internal	direct	0.0
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.7 ppm	internal	direct	0.0
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	H2O	protons	4.7 ppm	internal	direct	0.0
¹³C	H2O	protons	4.7 ppm	internal	indirect	0.2514495
¹⁵N	H2O	protons	4.7 ppm	internal	indirect	0.1013291

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Release date

2003-06-08

Citation 1

Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor
Sahasrabudhe, P.V., Stockman, B.J.
Biomol. NMR Assign. (2009), 3, 183-186, PubMed 19888686 , DOI 10.1007/s12104-009-9170-8 , Abstract

Show more 2 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 3

Automatic determination of protein backbone resonance assignments from triple resonance nuclear magnetic resonance data
Moseley, H.N., Monleon, D., Montelione, G.T.
Methods. Enzymol. (2001), 339, 91-108, PubMed 11462827 , DOI: , Abstract

Hide non-primary 2 citaions

Related entities 1. stromelysin, : 1 : 24 : 20 : 232 entities Detail

Interaction partners 1. stromelysin, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 9 experiments Detail

1 1H-15N HSQC