BMRBj - BMREntryMaster

Found 1 Document (1.488 seconds)

BMRB: 5543

1KZ2

1H chemical shifts for Penetratin(W48F,W56F) in phospolipid bicelles and SDS micelles

Authors

Lindberg, M., Biverstahl, H., Graslund, A., Maler, L.

Assembly

Penetratin(W48F,W56F)

Entity

1. Penetratin(W48F,W56F) (polymer, Thiol state: not present), 16 monomers, 2168.652 Da Detail

RQIKIFFQNR RMKFKK

Formula weight

2168.652 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 87.5 %, Completeness: 38.0 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	38.0 % (52 of 137)	38.0 % (52 of 137)
Backbone	84.4 % (27 of 32)	84.4 % (27 of 32)
Sidechain	23.8 % (25 of 105)	23.8 % (25 of 105)
Aromatic	0.0 % (0 of 15)	0.0 % (0 of 15)
Methyl	0.0 % (0 of 4)	0.0 % (0 of 4)

1. Penetratin(W48F,W56F)

RQIKIFFQNR RMKFKK

Show sample condition

Sample

Temperature 318 (±1) K, pH 5.5 (±0.1)

#	Name	Concentration
1	Penetratin(W48F,W56F)	2 mM
2	DMPC	10 mM
3	DMPG	90 mM
4	DHPC	192 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 40.9 %, Completeness (bb): 90.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	40.9 % (112 of 274)	40.9 % (112 of 274)
Backbone	90.6 % (58 of 64)	90.6 % (58 of 64)
Sidechain	25.7 % (54 of 210)	25.7 % (54 of 210)
Aromatic	0.0 % (0 of 30)	0.0 % (0 of 30)
Methyl	37.5 % (3 of 8)	37.5 % (3 of 8)

1. Penetratin(W48F,W56F)

RQIKIFFQNR RMKFKK

Show sample condition

Sample

Temperature 318 (±1) K, pH 3.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	Penetratin(W48F,W56F)			2 mM

Release date

2002-09-30

Citation

Structure and positioning comparison of two variants of penetratin in two different membrane mimicking systems by NMR
Lindberg, M., Biverstahl, H., Graslund, A., Maler, L.
Eur. J. Biochem. (2003), 270, 3055-3063, PubMed 12846839 , DOI: , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 5542 released on 2002-09-30
Title 1H chemical shifts for Penetratin in phospolipid bicelles

Related entities 1. Penetratin(W48F,W56F), : 1 : 160 : 1 : 113 entities Detail

Interaction partners 1. Penetratin(W48F,W56F), : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5543_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5543_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shifts_set_1	OK	44	87.5 (chain: 1, length: 16)
2	Assigned chemical shifts	chemical_shifts_set_2	Warning	66	100.0 (chain: 1, length: 16)
1	Chemical shift references	chemical_shift_reference	OK	1	No information

Assigned chemical shifts

Saveframe: chemical_shifts_set_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 16, Sequence coverage: 87.5%
- Total number of assignments
  - ¹H chemical shifts: 44
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: chemical_shifts_set_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 16, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 66
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.488 seconds)

BMRB: 5543

Sample

Sample

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Penetratin(W48F,W56F), : 1 : 160 : 1 : 113 entities Detail

Interaction partners 1. Penetratin(W48F,W56F), : 2 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail