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1H chemical shifts for Penetratin(W48F,W56F) in phospolipid bicelles and SDS micelles
Authors
Lindberg, M., Biverstahl, H., Graslund, A., Maler, L.
Assembly
Penetratin(W48F,W56F)
Entity
1. Penetratin(W48F,W56F) (polymer, Thiol state: not present), 16 monomers, 2168.652 Da Detail

RQIKIFFQNR RMKFKK


Formula weight
2168.652 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 87.5 %, Completeness: 32.1 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

Total1H
All32.1 % (44 of 137)32.1 % (44 of 137)
Backbone84.4 % (27 of 32)84.4 % (27 of 32)
Sidechain16.2 % (17 of 105)16.2 % (17 of 105)
Aromatic 0.0 % (0 of 15) 0.0 % (0 of 15)
Methyl 0.0 % (0 of 4) 0.0 % (0 of 4)

1. Penetratin(W48F,W56F)

RQIKIFFQNR RMKFKK

Sample

Temperature 318 (±1) K, pH 5.5 (±0.1)


#NameIsotope labelingTypeConcentration
1Penetratin(W48F,W56F)2 mM
2DMPC10 mM
3DMPG90 mM
4DHPC192 mM

Chem. Shift Complete2
Sequence coverage: 100.0 %, Completeness: 38.0 %, Completeness (bb): 90.6 % Detail

Polymer type: polypeptide(L)

Total1H
All38.0 % (104 of 274)38.0 % (104 of 274)
Backbone90.6 % (58 of 64)90.6 % (58 of 64)
Sidechain21.9 % (46 of 210)21.9 % (46 of 210)
Aromatic 0.0 % (0 of 30) 0.0 % (0 of 30)
Methyl37.5 % (3 of 8)37.5 % (3 of 8)

1. Penetratin(W48F,W56F)

RQIKIFFQNR RMKFKK

Sample

Temperature 318 (±1) K, pH 3.5 (±0.1)


#NameIsotope labelingTypeConcentration
5Penetratin(W48F,W56F)2 mM

Release date
2002-09-30
Citation
Structure and positioning comparison of two variants of penetratin in two different membrane mimicking systems by NMR
Lindberg, M., Biverstahl, H., Graslund, A., Maler, L.
Eur. J. Biochem. (2003), 270, 3055-3063, PubMed 12846839 , DOI 10.1046/j.1432-1033.2003.03685.x ,
Related entities 1. Penetratin(W48F,W56F), : 1 : 95 : 1 : 78 entities Detail
Interaction partners 1. Penetratin(W48F,W56F), : 2 interactors Detail
Experiments performed 2 experiments Detail