The Tertiary Structure and Backbone Dynamics of Human Prolactin: Evidence for Reversible Oligomerization in Solution
LPICPGGAAR CQVTLRDLFD RAVVLSHYIH NLSSEMFSEF DKRYTHGRGF ITKAINSCHT SSLATPEDKE QAQQMNQKDF LSLIVSILRS WNEPLYHLVT EVRGMQEAPE AILSKAVEIE EQTKRLLEGM ELIVSQVHPE TKENEIYPVW SGLPSLQMAD EESRLSAYYN LLHCLRRDSH KIDNYLKLLK CRIIHNNNC
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 0:CYS4:SG | 1:CYS11:SG |
2 | disulfide | sing | 0:CYS58:SG | 1:CYS174:SG |
3 | disulfide | sing | 0:CYS191:SG | 1:CYS199:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 67.7 % (1600 of 2362) | 59.4 % (733 of 1233) | 76.0 % (697 of 917) | 80.2 % (170 of 212) |
Backbone | 87.1 % (1026 of 1178) | 81.1 % (322 of 397) | 90.5 % (534 of 590) | 89.0 % (170 of 191) |
Sidechain | 54.0 % (743 of 1376) | 49.4 % (413 of 836) | 63.6 % (330 of 519) | 0.0 % (0 of 21) |
Aromatic | 8.4 % (14 of 166) | 8.4 % (7 of 83) | 8.6 % (7 of 81) | 0.0 % (0 of 2) |
Methyl | 68.1 % (154 of 226) | 69.0 % (78 of 113) | 67.3 % (76 of 113) |
1. Human Prolactin
LPICPGGAAR CQVTLRDLFD RAVVLSHYIH NLSSEMFSEF DKRYTHGRGF ITKAINSCHT SSLATPEDKE QAQQMNQKDF LSLIVSILRS WNEPLYHLVT EVRGMQEAPE AILSKAVEIE EQTKRLLEGM ELIVSQVHPE TKENEIYPVW SGLPSLQMAD EESRLSAYYN LLHCLRRDSH KIDNYLKLLK CRIIHNNNCTemperature 298 (±1) K, pH 6.8 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Human Prolactin | [U-13C; U-15N] | 1.0 mM | |
2 | phosphate buffer | 20 mM | ||
3 | NaCl | 100 mM |