Solution Structure of the 30S ribosomal protein S28E from Pyrococcus horikoshii. Northeast Structural Genomics Consortium target JR19
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.2 % (836 of 937) | 87.8 % (432 of 492) | 90.4 % (328 of 363) | 92.7 % (76 of 82) |
Backbone | 92.0 % (449 of 488) | 91.8 % (156 of 170) | 92.0 % (219 of 238) | 92.5 % (74 of 80) |
Sidechain | 87.0 % (455 of 523) | 85.7 % (276 of 322) | 88.9 % (177 of 199) | 100.0 % (2 of 2) |
Aromatic | 25.0 % (8 of 32) | 25.0 % (4 of 16) | 25.0 % (4 of 16) | |
Methyl | 100.0 % (102 of 102) | 100.0 % (51 of 51) | 100.0 % (51 of 51) |
1. RS28 PYRHO
MAEDEGYPAE VIEIIGRTGT TGDVTQVKVR ILEGRDKGRV IRRNVRGPVR VGDILILRET EREAREIKSR RAAALEHHHH HHTemperature 293 (±0.5) K, pH 6.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | RS28_PYRHO | [U-100% 13C; U-100% 15N] | 1.0 mM | |
2 | MES | 20 mM | ||
3 | NaCl | 100 mM | ||
4 | CaCl2 | 5 mM | ||
5 | DTT | 10 mM | ||
6 | NaN3 | 0.02 % | ||
7 | D2O | 5 % |