1H, 13C, and 15N backbone assignment of the first two Ig domains Z1Z2 of the giant muscle protein titin
MKHHHHHHPM ATQAPTFTQP LQSVVVLEGS TATFEAHISG FPVPEVSWFR DGQVISTSTL PGVQISFSDG RAKLTIPAVT KANSGRYSLK ATNGSGQATS TAELLVKAET APPNFVQRLQ SMTVRQGSQV RLQVRVTGIP TPVVKFYRDG AEIQSSLDFQ ISQEGDLYSL LIAEAYPEDS GTYSVNATNS VGRATSTAEL LVQG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.1 % (980 of 2274) | 28.7 % (335 of 1167) | 51.8 % (463 of 894) | 85.4 % (182 of 213) |
Backbone | 68.7 % (824 of 1200) | 54.7 % (225 of 411) | 70.2 % (419 of 597) | 93.8 % (180 of 192) |
Sidechain | 26.5 % (335 of 1263) | 14.6 % (110 of 756) | 45.9 % (223 of 486) | 9.5 % (2 of 21) |
Aromatic | 3.8 % (6 of 160) | 3.8 % (3 of 80) | 3.8 % (3 of 79) | 0.0 % (0 of 1) |
Methyl | 28.3 % (69 of 244) | 23.0 % (28 of 122) | 33.6 % (41 of 122) |
1. titin Z1Z2
MKHHHHHHPM ATQAPTFTQP LQSVVVLEGS TATFEAHISG FPVPEVSWFR DGQVISTSTL PGVQISFSDG RAKLTIPAVT KANSGRYSLK ATNGSGQATS TAELLVKAET APPNFVQRLQ SMTVRQGSQV RLQVRVTGIP TPVVKFYRDG AEIQSSLDFQ ISQEGDLYSL LIAEAYPEDS GTYSVNATNS VGRATSTAEL LVQGTemperature 310 (±1) K, pH 5.7 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | titin Z1Z2 | [U-100% 15N; U-100% 13C; U-70% 2H] | 1.0 mM | |
2 | sodium acetate | 10 mM | ||
3 | (NH4)2SO4 | 50 mM |