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1H, 15N and 13C resonance assignments of the ApaG/CorD protein of the phytopathogen Xanthomonas axonopodis pv. citri
Authors
Katsuyama, A.M., Cicero, D.O., Spisni, A., Paci, M., Farah, C.S., Pertinhez, T.A.
Assembly
ApaG/CorD protein
Entity
1. ApaG/CorD protein (polymer, Thiol state: not present), 127 monomers, 14181.56 Da Detail

MQDDPRYRVE VEVSPRFLAH QSTPDEGRYA FAYSIRIQNA GAVPARLVAR HWQITDGNGR TEQVDGEGVV GEQPWLRPGE AFHYTSGVLL ETEQGQMQGH YDMVADDGTE FIAPIAAFVL SVPRTLH


Formula weight
14181.56 Da
Source organism
Xanthomonas axonopodis
Exptl. method
NMR
Refine. method
Simulated Annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 89.9 %, Completeness (bb): 96.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All89.9 % (1291 of 1436)89.1 % (661 of 742)92.5 % (520 of 562)83.3 % (110 of 132)
Backbone96.0 % (716 of 746)95.3 % (246 of 258)97.8 % (361 of 369)91.6 % (109 of 119)
Sidechain85.3 % (687 of 805)85.7 % (415 of 484)88.0 % (271 of 308) 7.7 % (1 of 13)
Aromatic64.2 % (86 of 134)67.2 % (45 of 67)61.5 % (40 of 65)50.0 % (1 of 2)
Methyl100.0 % (134 of 134)100.0 % (67 of 67)100.0 % (67 of 67)

1. ApaG protein

MQDDPRYRVE VEVSPRFLAH QSTPDEGRYA FAYSIRIQNA GAVPARLVAR HWQITDGNGR TEQVDGEGVV GEQPWLRPGE AFHYTSGVLL ETEQGQMQGH YDMVADDGTE FIAPIAAFVL SVPRTLH

Sample

Temperature 303 (±1) K, pH 6.8 (±0.2)


#NameIsotope labelingTypeConcentration
1ApaG protein[U-100% 13C; U-100% 15N]0.5 mM
2NaCl50 mM
3NaH2PO440 mM

LACS Plot; CA
Referencing offset: 0.82 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 0.82 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.15 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.48 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 2F1E, Strand ID: A Detail


Release date
2004-07-05
Citation 1
1H, 15N and 13C resonance assignments of the ApaG protein of the phytopathogen Xanthomonas axonopodis pv. citri
Katsuyama, A.M., Cicero, D.O., Spisni, A., Paci, M., Farah, C.S., Pertinhez, T.A.
J. Biomol. NMR (2004), 29, 423-424, PubMed 15213450 , DOI 10.1023/B:JNMR.0000032502.66997.25 ,
Related entities 1. ApaG/CorD protein, : 1 : 1 : 1 : 118 entities Detail
Experiments performed 12 experiments Detail
nullKeywords ApaG/CorD protein, Xanthomonas axonopodis pv. citri, NMR