BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	88.8 % (1195 of 1345)	87.6 % (614 of 701)	89.5 % (471 of 526)	93.2 % (110 of 118)
Backbone	93.3 % (616 of 660)	94.2 % (210 of 223)	93.0 % (306 of 329)	92.6 % (100 of 108)
Sidechain	85.7 % (679 of 792)	84.5 % (404 of 478)	87.2 % (265 of 304)	100.0 % (10 of 10)
Aromatic	53.1 % (51 of 96)	56.3 % (27 of 48)	50.0 % (24 of 48)
Methyl	94.9 % (129 of 136)	95.6 % (65 of 68)	94.1 % (64 of 68)

1. Carnobacteriocin B2 immunity protein

MDIKSQTLYL NLSEAYKDPE VKANEFLSKL VVQCAGKLTA SNSENSYIEV ISLLSRGISS YYLSHKRIIP SSMLTIYTQI QKDIKNGNID TEKLRKYEIA KGLMSVPYIY F

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Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 1TDP, Strand ID: A Detail

Coupling constant

71 J values in 1 lists
Temperature 288 (±0.1) K, pH 6.6 (±0.1) Detail

Release date

2004-11-11

Citation 1

NMR solution structure of ImB2, a protein conferring immunity to antimicrobial activity of the type IIa bacteriocin, carnobacteriocin B2
Sprules, T., Kawulka, K.E., Vederas, J.C.
Biochemistry (2004), 43, 11740-11749, PubMed 15362858 , DOI 10.1021/bi048854+ , Abstract

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Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

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Related entities 1. Carnobacteriocin B2 immunity protein, : 1 : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

1 15N-HSQC NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

2 15N-HSQC TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

3 13C-HSQC NOESY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

4 13C-HSQC TOCSY

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

5 HNHA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

6 13C-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

7 HNCACB

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

8 HNCA

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

9 HNCO

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

10 CBCACONH

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

11 15N-HSQC

Instrument

#1 Model Varian INOVA (600 MHz)

#2 Model Varian INOVA (800 MHz)

Sample #1

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	Carnobacteriocin B2 immunity protein	[U-15N]	0.8 mM
2	DSS		0.025 mM
3	sodium phosphate		20 mM
4	sodium chloride		25 mM

Sample #2

Temperature 288 (±0.1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	Carnobacteriocin B2 immunity protein	[U-13C; U-15N]	0.8 mM
6	DSS		0.025 mM
7	sodium phosphate		20 mM
8	sodium chloride		25 mM

nullKeywords immunity protein, bacteriocin

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BMRB: 6211

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Related entities 1. Carnobacteriocin B2 immunity protein, : 1 : 1 : 1 entities Detail

Experiments performed 11 experiments Detail

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