BMRB: 6243

NMR Relaxation data for Protein Azurin from Pseudomonas aeruginosa

Authors

Zhuravleva, A.V., Korzhnev, D.M., Kupce, E., Billeter, M., Arseniev, A.S., Orekhov, V.YU.

Assembly

azurin

Entity

1. azurin (polymer, Thiol state: disulfide and other bound), 128 monomers, 13945.66 Da Detail

AECSVDIQGN DQMQFNTNAI TVDKSCKQFT VNLSHPGNLP KNVMGHNWVL STAADMQGVV TDGMASGLDK DYLKPDDSRV IAHTKLIGSG EKDSVTFDVS KLKEGEQYMF FCTFPGHSAL MKGTLTLK

2. CU1 (non-polymer), 63.546 Da

Total weight

14009.206 Da

Max. entity weight

13945.66 Da

Entity Connection

na 1, disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS26:SG
2	na	sing	1:CYS112:SG	2:CU11:CU

Source organism

Pseudomonas aeruginosa

Exptl. method

NMR

Data set

heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation, order_parameters

Heteronucl. T₁

806 T₁ values in 7 lists
Coherence Sz, Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, 900 MHz, Temperature 289 (±0.2) K, 317 (±0.2) K, pH 5.5 (±0.2) Detail

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Temperature 289 (±0.2) K, pH 5.5 (±0.2)

Temperature 317 (±0.2) K, pH 5.5 (±0.2)

Heteronucl. T₂

806 T₂ values in 7 lists
Coherence S(+,-), Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, 900 MHz, Temperature 289 (±0.2) K, 317 (±0.2) K, pH 5.5 (±0.2) Detail

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Temperature 289 (±0.2) K, pH 5.5 (±0.2)

Temperature 317 (±0.2) K, pH 5.5 (±0.2)

Heteronucl. NOE

690 NOE values in 6 lists
Value type relative intensities, Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, Temperature 289 (±0.2) K, 317 (±0.2) K, pH 5.5 (±0.2) Detail

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Temperature 289 (±0.2) K, pH 5.5 (±0.2)

Temperature 317 (±0.2) K, pH 5.5 (±0.2)

Heteronucl. T₁/T₂

806 T₁/T₂ values in 7 lists
Field strength (¹H) 500 MHz, 600 MHz, 800 MHz, 900 MHz, Temperature 289 (±0.2) K, 317 (±0.2) K, pH 5.5 (±0.2) Detail

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Temperature 289 (±0.2) K, pH 5.5 (±0.2)

Temperature 317 (±0.2) K, pH 5.5 (±0.2)

Order parameters

230 S² values in 2 lists
Temperature 289 (±0.2) K, 317 (±0.2) K, pH 5.5 (±0.2) Detail

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Temperature 289 (±0.2) K, pH 5.5 (±0.2)

List #1 S2_parameters_1

Temperature 317 (±0.2) K, pH 5.5 (±0.2)

List #2 S2_parameters_2

Release date

2004-09-19

Citation 1

Gated electron transfers and electron pathways in azurin: a NMR dynamic study at multiple fields and temperatures
Zhuravleva, A.V., Korzhnev, D.M., Kupce, E., Arseniev, A.S., Billeter, M., Orekhov, V.YU.
J. Mol. Biol. (2004), 342, 1599-1611, PubMed 15364584 , DOI 10.1016/j.jmb.2004.08.001 , Abstract

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Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 3

MUNIN: application of three-way decomposition to the analysis of heteronuclear NMR relaxation data
Korzhneva, D.M., Ibraghimov, I.V., Billeter, M., Orekhov, V.Y.
J. Biomol. NMR (2001), 21, 263-268, PubMed 11775742 , Abstract

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Related entities 1. azurin, : 1 : 19 : 8 : 38 : 57 entities Detail

Experiments performed 3 experiments Detail