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Solution Structure of the Conserved Protein from the Gene Locus Mm1357 of Methanosarcina Mazei. Northeast Structural Genomics Target Mar30
Authors
Rossi, P., Aramini, J.M., Swapna, G.V.T., Huang, Y.P., Xiao, R., Ho, C.K., Ma, L.C., Acton, T.B., Montelione, G.T.
Assembly
hypothetical protein MM1357
Entity
1. hypothetical protein MM1357 (polymer, Thiol state: not present), 68 monomers, 7611.594 Da Detail

MFREESRSVP VEEGEVYDVT IQDIARQGDG IARIEGFVIF VPGTKVGDEV RIKVERVLPK FAFASVVE


Formula weight
7611.594 Da
Source organism
Methanosarcina mazei
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 95.2 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All95.2 % (753 of 791)93.7 % (384 of 410)97.1 % (305 of 314)95.5 % (64 of 67)
Backbone97.0 % (390 of 402)95.7 % (133 of 139)98.5 % (195 of 198)95.4 % (62 of 65)
Sidechain94.0 % (424 of 451)92.6 % (251 of 271)96.1 % (171 of 178)100.0 % (2 of 2)
Aromatic86.2 % (50 of 58)86.2 % (25 of 29)86.2 % (25 of 29)
Methyl100.0 % (88 of 88)100.0 % (44 of 44)100.0 % (44 of 44)

1. MM1357

MFREESRSVP VEEGEVYDVT IQDIARQGDG IARIEGFVIF VPGTKVGDEV RIKVERVLPK FAFASVVE

Sample

Pressure 1 atm, Temperature 293 K, pH 6.5


#NameIsotope labelingTypeConcentration
1MM1357[U-13C; U-15N]2 mM
2NaN30.02 %
3DTT10 mM
4CaCl25 mM
5NaCl100 mM
6MES20 mM
7D2O5 %
8H2O95 %

LACS Plot; CA
Referencing offset: -0.27 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.27 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.13 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: 0.42 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 10 models in PDB: 1YEZ, Strand ID: A Detail


Release date
2005-12-08
Citation 1
Microgram-scale protein structure determination by NMR
Aramini, J.M., Rossi, P., Anklin, C., Xiao, R., Montelione, G.T.
Nat. Methods (2007), 4, 491-493, PubMed 17496898 , DOI 10.1038/nmeth1051 ,
Citation 2
NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 ,
Related entities 1. hypothetical protein MM1357, : 1 : 69 entities Detail
Experiments performed 17 experiments Detail
nullKeywords MaR30, NMR Structure, Autostructure, Northeast Structural Genomics, PSI, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG