Solution Structure of the Conserved Protein from the Gene Locus Mm1357 of Methanosarcina Mazei. Northeast Structural Genomics Target Mar30
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.2 % (753 of 791) | 93.7 % (384 of 410) | 97.1 % (305 of 314) | 95.5 % (64 of 67) |
Backbone | 97.0 % (390 of 402) | 95.7 % (133 of 139) | 98.5 % (195 of 198) | 95.4 % (62 of 65) |
Sidechain | 94.0 % (424 of 451) | 92.6 % (251 of 271) | 96.1 % (171 of 178) | 100.0 % (2 of 2) |
Aromatic | 86.2 % (50 of 58) | 86.2 % (25 of 29) | 86.2 % (25 of 29) | |
Methyl | 100.0 % (88 of 88) | 100.0 % (44 of 44) | 100.0 % (44 of 44) |
1. MM1357
MFREESRSVP VEEGEVYDVT IQDIARQGDG IARIEGFVIF VPGTKVGDEV RIKVERVLPK FAFASVVEPressure 1 atm, Temperature 293 K, pH 6.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MM1357 | [U-13C; U-15N] | 2 mM | |
2 | NaN3 | 0.02 % | ||
3 | DTT | 10 mM | ||
4 | CaCl2 | 5 mM | ||
5 | NaCl | 100 mM | ||
6 | MES | 20 mM | ||
7 | D2O | 5 % | ||
8 | H2O | 95 % |