BMRBj - BMREntryMaster

Found 1 Document (1.358 seconds)

BMRB: 6648

1ZW8

Solution Structure of a Zap1 Zinc-Responsive Domain Provides Insights into Metalloregulatory Transcriptional Repression in Saccharomyces cerevisiae

Authors

Wang, Z., Feng, L.S., Venkataraman, K., Matskevich, V.A., Parasuram, P., Laity, J.H.

Assembly

Zap1-monomer

Entity

1. Zap1-2 (polymer, Thiol state: all other bound), 64 monomers, 7408.192 Da Detail

DLKCKWKECP ESASSLFDLQ RHLLKDHVSQ DFKHPMEPLA CNWEDCDFLG DDTASIVNHI NAQH

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

7539.01 Da

Max. entity weight

7408.192 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS4:SG	2:ZN1:ZN
2	na	sing	1:CYS9:SG	2:ZN1:ZN
3	na	sing	1:HIS22:NE2	2:ZN1:ZN
4	na	sing	1:HIS27:NE2	2:ZN1:ZN
5	na	sing	1:CYS41:SG	2:ZN1:ZN
6	na	sing	1:CYS46:SG	2:ZN1:ZN
7	na	sing	1:HIS59:NE2	2:ZN1:ZN
8	na	sing	1:HIS64:NE2	2:ZN1:ZN

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.0 %, Completeness (bb): 98.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.0 % (704 of 749)	94.4 % (368 of 390)	93.4 % (271 of 290)	94.2 % (65 of 69)
Backbone	98.1 % (371 of 378)	96.8 % (122 of 126)	99.5 % (190 of 191)	96.7 % (59 of 61)
Sidechain	91.2 % (396 of 434)	93.2 % (246 of 264)	88.9 % (144 of 162)	75.0 % (6 of 8)
Aromatic	86.5 % (64 of 74)	89.2 % (33 of 37)	88.6 % (31 of 35)	0.0 % (0 of 2)
Methyl	98.1 % (53 of 54)	100.0 % (27 of 27)	96.3 % (26 of 27)

1. Zap1-2

DLKCKWKECP ESASSLFDLQ RHLLKDHVSQ DFKHPMEPLA CNWEDCDFLG DDTASIVNHI NAQH

Show sample condition

Sample

Temperature 293 (±0.1) K, pH 6.9 (±0.03), Details Zap1-2, Zn(II), MES, DSS, NaN2, BME

#	Name	Isotope labeling	Concentration
1	Zap1-2	[U-13C; U-15N]	0.7 mM
2	ZN		1.5 mM
3	MES		20 mM
4	BME		5 mM
5	DSS		0.2 mM
6	NaN2		1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.65 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.65 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 1 Detail

Release date

2006-04-26

Citation 1

Zinc fingers can act as Zn(II) sensors to regulate transcriptional activation domain function
Bird, A.J., McCall, K., Kramer, M., Blankman, E., Winge, D.R., Eide, D.J.
EMBO J. (2003), 22, 5137-5146, PubMed , DOI:

Show more 4 citaions

Citation 2

Zap1p, a metalloregulatory protein involved in zinc-responsive transcriptional regulation in Saccharomyces cerevisiae
Zhao, H., Eide, D.J.
Mol. Cell. Biol. (1997), 17, 5044-5052, PubMed 9271382 , DOI: , Abstract

Citation 3

Solution structure of a Zap1 zinc-responsive domain provides insights into metalloregulatory transcriptional repression in Saccharomyces cerevisiae
Wang, Z., Feng, L.S., Matskevich, V.A., Venkataraman, K., Parasuram, P., Laity, J.J.
J. Mol. Biol. (2006), 357, 1167-1183, PubMed 16483601 , DOI 10.1016/j.jmb.2006.01.010 , Abstract

Citation 4

NMRView: A computer program for the visualization and analysis of NMR Data
Johnson, B.A., Blevins, R.A.
J. Biomol. NMR (1994), 4, 603-614, PubMed , DOI:

Citation 5

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 4 citaions

Related entities 1. Zap1-2, : 1 : 2 : 82 entities Detail

Interaction partners 1. Zap1-2, : 7 interactors Detail

More details for 7 interactors identified by 6 citations