NMR assignment of the E.coli cytolethal distending toxin CdtB-II subunit
MAHHHHHHVG TDLTDFRVAT WNLQGASATT ESKWNINVRQ LISGENAVDI LAVQEAGSPP STAVDTGRVI PSPGIPVREL IWNLSTNSRP QQVYIYFSAV DALGGRVNLA LVSNRRADEV FVLSPVRQGG RPLLGIRIGN DAFFTAHAIA MRNNDAPALV EEVYNFFRDS RDPVHQALNW MILGDFNREP ADLEMNLTVP VRRASEIISP AAATQTSQRT LDYAVAGNSV AFRPSPLQAG IVYGARRTQI SSDHFPVGVS RR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 38.5 % (1136 of 2948) | 18.8 % (285 of 1518) | 56.1 % (647 of 1153) | 73.6 % (204 of 277) |
Backbone | 68.4 % (1053 of 1540) | 42.7 % (224 of 525) | 81.3 % (625 of 769) | 82.9 % (204 of 246) |
Sidechain | 17.6 % (291 of 1653) | 6.1 % (61 of 993) | 36.6 % (230 of 629) | 0.0 % (0 of 31) |
Aromatic | 1.8 % (4 of 224) | 1.8 % (2 of 112) | 1.9 % (2 of 108) | 0.0 % (0 of 4) |
Methyl | 22.4 % (72 of 322) | 16.1 % (26 of 161) | 28.6 % (46 of 161) |
1. EcCdtB-II
MAHHHHHHVG TDLTDFRVAT WNLQGASATT ESKWNINVRQ LISGENAVDI LAVQEAGSPP STAVDTGRVI PSPGIPVREL IWNLSTNSRP QQVYIYFSAV DALGGRVNLA LVSNRRADEV FVLSPVRQGG RPLLGIRIGN DAFFTAHAIA MRNNDAPALV EEVYNFFRDS RDPVHQALNW MILGDFNREP ADLEMNLTVP VRRASEIISP AAATQTSQRT LDYAVAGNSV AFRPSPLQAG IVYGARRTQI SSDHFPVGVS RRTemperature 310 (±0.2) K, pH 6.0 (±0.2), Details 20 mM sodium phosphate,50 mM NaCl, 0.02% Na3N, 0.2 mM DSS, 5% D2O, ~0.4mM rEcCdtB-II
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | EcCdtB-II | [U-13C; U-15N; U-2H] | protein | 0.3 ~ 0.5 mM |
2 | Sodium phosphate | 20 mM | ||
3 | Sodium Chloride | 50 mM | ||
4 | DSS | internal reference | 0.2 mM | |
5 | D2O | 5 % v/v | ||
6 | Sodium azide | 0.02 % w/v |