Chemical Shift Assignments for V66W110 fragment of Staphylococcal Nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 65.6 % (531 of 809) | 63.0 % (440 of 698) | 82.0 % (91 of 111) |
Backbone | 86.9 % (286 of 329) | 87.5 % (196 of 224) | 85.7 % (90 of 105) |
Sidechain | 51.0 % (245 of 480) | 51.5 % (244 of 474) | 16.7 % (1 of 6) |
Aromatic | 60.9 % (28 of 46) | 60.0 % (27 of 45) | 100.0 % (1 of 1) |
Methyl | 49.1 % (28 of 57) | 49.1 % (28 of 57) |
1. V66W110 fragment of staphylococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMWENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAKTemperature 298 (±0.1) K, pH 4.9 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | V66W110 fragment of staphylococcal nuclease | [U-95% 15N] | 1.0 ~ 2.5 mM | |
2 | deuterated acetate | [U-99% 2H] | 50 (±1.0) mM |