BMRB: 7139

1H,13C and 15N resonance assignments of barnase-barstar complex NMR deuterium methyl relaxation data for free barnase and barnase-barstar complex

Authors

Zhuravleva, A.V., Korzhnev, D.M., Nolde, S.B., Kay, L.E., Arseniev, A.S., Billiter, M., Orekhov, V.YU.

Assembly

free barnase

Entity

1. free barnase (polymer, Thiol state: not present), 110 monomers, 12382.59 Da Detail

AQVINTFDGV ADYLQTYHKL PDNYITKSEA QALGWVASKG NLADVAPGKS IGGDIFSNRE GKLPGKSGRT WREADINYTS GFRNSDRILY SSDWLIYKTT DHYQTFTKIR

Formula weight

12382.59 Da

Source organism

Bacillus amyloliquefaciens

Exptl. method

NMR

Data set

order_parameters

Order parameters

41 S² values in 1 lists
Temperature 303 (±0.2) K, pH 6.7 (±0.5) Detail

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Temperature 303 (±0.2) K, pH 6.7 (±0.5)

Experiment name 13C HSQC-RQ(3Dz2-2), 13C HSQC-RQ(D+), 13C HSQC-RQ(D+z+Dz+), 13C HSQC-RQ(Dz)

List #1 S2_free, Details Methyl axial order parameters of sub-nanosecond motions for free barnase

Release date

2007-03-05

Citation 1

Propagation of dynamic changes in barnase upon binding of barstar: an NMR and computational study
Zhuravleva, A.V., Korzhnev, D.M., Nolde, S.B., Kay, L.E., Arseniev, A.S., Billiter, M., Orekhov, V.YU.
J. Mol. Biol. (2007), 367, 1079-1092, PubMed 17306298 , DOI 10.1016/j.jmb.2007.01.051 , Abstract

Show more 4 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Citation 3

Processing of heteronuclear NMR relaxation data with the new software DASHA
Orekhov, V.YU., Nolde, D.E., Golovanov, A.P., Korzhnev, D.M., Arseniev, A.S.
Appl. Magn. Reson. (1995), 9, 581-588, PubMed , DOI:

Citation 4

optimizing the process of nuclear magnetic resonance spectrum analysis and computer aided resonance assigment
Keller, R.

Citation 5

Deuterium spin probes of side-chain dynamics in proteins. 2. Spectral density mapping and identification of nanosecond time-scale side-chain motions
Millet, O.D., Muhandiram, N.R., Skrynnikov, N.R., Kay, L.E.
J. Am. Chem. Soc. (2002), 124, 6449-6460, PubMed 12033876 , DOI: , Abstract

Hide non-primary 4 citaions

Related entities 1. free barnase, : 1 : 15 : 9 : 26 : 12 entities Detail

Interaction partners 1. free barnase, : 1 interactors Detail

Experiments performed 9 experiments Detail