BMRBj - BMREntryMaster

Found 1 Document (1.186 seconds)

BMRB: 7277

Backbone amide chemical shifts for dG85 T. thermophilus RNase H

Authors

Butterwick, J.A., Palmer, A.G.

Assembly

dG85 ttRNH

Entity

1. dG85 ttRNH (polymer, Thiol state: not present), 165 monomers, 18574.85 Da Detail

MNPSPRKRVA LFTDGAALGN PGPGGWAALL RFHAHEKLLS GGEASTTNNR MELKAAIEGL KALKEPAEVD LYTDSHYLKK AFTEWLEGWR KRGWRTAEGK PVKNRDLWEA LLLAMAPHRV RFHFVKGHTG HPENERVDRE ARRQAQSQAK TPSPPRAPTL FHEEA

Formula weight

18574.85 Da

Source organism

Thermus thermophilus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.9 %, Completeness: 27.6 %, Completeness (bb): 62.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	27.6 % (327 of 1183)	17.9 % (182 of 1016)	86.8 % (145 of 167)
Backbone	62.2 % (301 of 484)	47.1 % (156 of 331)	94.8 % (145 of 153)
Sidechain	3.9 % (27 of 699)	3.9 % (27 of 685)	0.0 % (0 of 14)
Aromatic	0.0 % (0 of 89)	0.0 % (0 of 84)	0.0 % (0 of 5)
Methyl	2.6 % (2 of 78)	2.6 % (2 of 78)

1. dG85 ttRNH

MNPSPRKRVA LFTDGAALGN PGPGGWAALL RFHAHEKLLS GGEASTTNNR MELKAAIEGL KALKEPAEVD LYTDSHYLKK AFTEWLEGWR KRGWRTAEGK PVKNRDLWEA LLLAMAPHRV RFHFVKGHTG HPENERVDRE ARRQAQSQAK TPSPPRAPTL FHEEA

Show sample condition

Sample

Temperature 300 (±1) K, pH 5.5 (±0.1), Details 100 mM sodium acetate-d3, pH 5.5, 1 mM DSS, 0.02% (w/v) NaN3, 10% (v/v) D2O

#	Name	Isotope labeling	Concentration
1	dG85 ttRNH	[U-2H; U-15N]	0.3 mM
2	sodium acetate-d3		100 mM
3	DSS		1 mM
4	NaN3		0.02 % w/v
5	D2O		10 %
6	H2O		90 %

Release date

2007-10-08

Citation

An inserted Gly residue fine tunes dynamics between mesophilic and thermophilic ribonucleases H
Butterwick, J.A., Palmer, A.G.
Protein Sci. (2006), 15, 2697-2707, PubMed 17088323 , DOI 10.1110/ps.062398606 , Abstract

Related entities 1. dG85 ttRNH, : 168 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr7277_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr7277_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chem_shift_list_1	Warning	288		87.3 (chain: 1, length: 165)
1	Chemical shift references	chemical_shift_referencing	OK	2		No information

Assigned chemical shifts

Saveframe: chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 165, Sequence coverage: 87.3%
- Total number of assignments
  - ¹H chemical shifts: 144
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (1.186 seconds)

BMRB: 7277

Sample

Related entities 1. dG85 ttRNH, : 168 entities Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1016	144	14.2
¹⁵N chemical shifts	182	144	79.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	331	144	43.5
¹⁵N chemical shifts	153	144	94.1